| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067604 |
| Chromosome: | chromosome 2 |
| Location: | 4164243 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103850 | HIS3 | (1 of 1) 4.2.1.19 - Imidazoleglycerol-phosphate dehydratase; Imidazoleglycerol-phosphate dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACGAACGAGACTCAAACATTCCCAAAAGCCCCACACCTCGTGCTCAA |
| Internal bar code: | GATTAAAACTCACCCACCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2623 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATCGGGAATGGAACCCCC |
| Suggested primer 2: | CTGTTGATGGGCCTCGACTT |