Insertion junction: CLIP2.067607_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.067607
Chromosome:chromosome 7
Location:148442
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g313122 (1 of 2) 3.4.19.1 - Acylaminoacyl-peptidase / N-acylpeptide hydrolase 3'UTR
Cre07.g313143 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGATATCGATCGCGACTGACATGGGCTGATGCATTCTTGTAAGGCGGC
Internal bar code:ATGGCAATACTTGGTGATGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGTCCTCCTGCTGACTT
Suggested primer 2:AACTGACTCTCGGTGCCATG