| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067610 |
| Chromosome: | chromosome 12 |
| Location: | 3223854 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499800 | ASA10 | Mitochondrial F1F0 ATP synthase associated protein 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACAACGAGACGAAGCCGTAGGTATGCTGGCGGCCTGGTCAGATGATAC |
| Internal bar code: | AGGATTTGGGACTAGGGTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3171 |
| LEAP-Seq percent confirming: | 92.4528 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACGCTCGGGCTTATCGT |
| Suggested primer 2: | GACGGCACTAGATACAGGCC |