| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067623 |
| Chromosome: | chromosome 14 |
| Location: | 877809 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613134 | (1 of 11) 2.1.1.259 - [Fructose-bisphosphate aldolase]-lysine N-methyltransferase / (dimerizing)]-lysine 6-N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGGTGCGCCAGTCTGCATCAGCCCCGGTCAGAAGCCAGGCGGACGG |
| Internal bar code: | CAGTAATCCTTTACAAGACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTTGGGATTGCCAGTGA |
| Suggested primer 2: | ATCGCTTGCAACTAAGGCCT |