| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067634 |
| Chromosome: | chromosome 1 |
| Location: | 689519 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003800 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; MAP kinase kinase kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCCGTGCGTATGGTAGTTGCTTTATTAACTCCTGAGAAGCGGAAGGT |
| Internal bar code: | CATTAGAGTGGGGGTTGTCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4467 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 159 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGCGGGAATATGGCTTT |
| Suggested primer 2: | ACACTCAGCGAAATGACGGT |