| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067638 |
| Chromosome: | chromosome 16 |
| Location: | 885408 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801886 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGAGGGAGGAGGGAGGAGCCGGACGGCAGGGGGGAGGAGCAGGGTT |
| Internal bar code: | CCGTCAATGTTATTCTTACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACACGTCGTAACCACCT |
| Suggested primer 2: | CTGCGTTGCATGAACACCAA |