| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067639 |
| Chromosome: | chromosome 17 |
| Location: | 2549558 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715950 | (1 of 2) PTHR12775:SF0 - PROTEIN RTF2 HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCTCGGAAGCCTGCCGGAAGCCTCAGGAGCCTAACGGGTCCTGTCT |
| Internal bar code: | GGTGTGAAGATCGAGCAGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 24.0741 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAACTGCTCGGTGTGTGG |
| Suggested primer 2: | AAGAGGAGGAGGAGCAGGAG |