| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067661 |
| Chromosome: | chromosome 12 |
| Location: | 3423880 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497700 | SNE8 | (1 of 8) PTHR10366:SF411 - HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTAAGCCACTGTGCATCTTGCGCACAGAACTGCAGGCCCGTACTAGA |
| Internal bar code: | GTATGTGATCTGGTTGACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5437 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACCCGAACCTCAGCAAG |
| Suggested primer 2: | CTCTGGCGATGGTTTGCATG |