Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.067665 |
Chromosome: | chromosome 3 |
Location: | 5570520 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g185400 | MAW2 | Membrane-associated hydroxyproline-rich glycoprotein 2 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGCTCGGCAGCTCCACCATAAAACCGTCTGTGGCGAAGGCTGGGGGCA |
Internal bar code: | ATTTCGTTAACCTGACTAGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 588 |
LEAP-Seq percent confirming: | 72.2222 |
LEAP-Seq n confirming: | 13 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCCTACAACAACACCTCCA |
Suggested primer 2: | AAGCATTAGGCCAGCCACAT |