Insertion junction: CLIP2.067676_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067676
Chromosome:chromosome 1
Location:986933
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g005750 (1 of 11) IPR002350 - Kazal domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGGGGGGATCATCTTTTAGTAAGTTGTACAGCTTGATGGTGTGCGCAT
Internal bar code:TCGCTATAGGGATCGACAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:6146
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:86
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGCATCTAGGGGCACAA
Suggested primer 2:GCTCAAGCATTAGCATGGCC