| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067684 |
| Chromosome: | chromosome 10 |
| Location: | 5478687 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTCGCTGTATCAATCATACAACGCAGCCAAAACTGGGTGCTCTCTTC |
| Internal bar code: | CGGTGGGGGTTTTTGGAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3145 |
| LEAP-Seq percent confirming: | 30.5263 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCATGCCCACATCAGTG |
| Suggested primer 2: | CTGCACGATGTTGGAGTCCT |