| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067695 |
| Chromosome: | chromosome 13 |
| Location: | 3503966 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587550 | FXL3 | FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) | 3'UTR |
| Cre13.g587600 | (1 of 2) PTHR10907//PTHR10907:SF33 - REGUCALCIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCATGGCTGTGTCCATGCTGCCAGGGTCAGTCCTGGGTAGCTGGTG |
| Internal bar code: | CGATGTGTAAGGGACAGCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2347 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCCCAAGAACTAACCGT |
| Suggested primer 2: | CGCGCTACATGTTCATGGTG |