Insertion junction: CLIP2.067695_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067695
Chromosome:chromosome 13
Location:3503968
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g587550 FXL3 FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) 3'UTR
Cre13.g587600 (1 of 2) PTHR10907//PTHR10907:SF33 - REGUCALCIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGATATGGTACTTGCGCGAAACCCCTTGCGCGGGAACATCTCAGACC
Internal bar code:GAGATTTGTTAGAGGTCAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1047
LEAP-Seq percent confirming:20.9302
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGCTACATGTTCATGGTG
Suggested primer 2:CCTGCCCAAGAACTAACCGT