| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067700 |
| Chromosome: | chromosome 6 |
| Location: | 8478982 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308900 | SAM50,TOB55 | Sorting assembly machinery 50 kDa subunit; (1 of 2) K07277 - outer membrane protein insertion porin family (SAM50, TOB55, bamA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCACGTGGTGAGTGGGGTTCTAGGCAGGTAGAGGGAAGTTGCACGGG |
| Internal bar code: | GAGCTGGAGGAGACTGGAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTCACAGCCTGTCCTC |
| Suggested primer 2: | GGGGGTTACTAGGCCAAACC |