| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067705 |
| Chromosome: | chromosome 5 |
| Location: | 542623 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244350 | outside_mRNA | ||
| Cre05.g244400 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACAGGCGTGAATATGGCGCGCAAACAAGCATACCGACGCATGAAAGC |
| Internal bar code: | GGAATCCCAAAGGAATGAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2423 |
| LEAP-Seq percent confirming: | 57.8947 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGGTGCTAAGCTGAGG |
| Suggested primer 2: | AGTGGGTGGGCTATCTGCTA |