Insertion junction: CLIP2.067707_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067707
Chromosome:chromosome 8
Location:465764
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g359000 (1 of 32) IPR006502 - Protein of unknown function PDDEXK-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCTCGCGCTTGTTTGCGCTCAGCTATAGTCGACCCGCTCACCCACA
Internal bar code:ATGGGATTTCTTGATGAAATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2948
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGGGTGTTTATGTGATGC
Suggested primer 2:CTGTGTGACTGGCAGTGCTA