| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067708 |
| Chromosome: | chromosome 8 |
| Location: | 2968463 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATATACCGTAGCACAGAACCGAGCTCCATTCGTGTCAGGTCCCCCCT |
| Internal bar code: | GTCACATGAAACGGAGTTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1925 |
| LEAP-Seq percent confirming: | 95.3846 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGGCCGTAGATAGAGTTT |
| Suggested primer 2: | GTGGCTAATGTGGGTCAGCT |