| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067722 |
| Chromosome: | chromosome 16 |
| Location: | 1338705 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651750 | TSI,TSI2 | isoleucyl-tRNA synthetase; (1 of 2) K01870 - isoleucyl-tRNA synthetase (IARS, ileS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAATGACAGCTAAAGCAGGATGTCTCAAATGCGACTATGCAGAGAAG |
| Internal bar code: | GAACGCTCTTTCTAATCCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5498 |
| LEAP-Seq percent confirming: | 58.0247 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCCCACCAGCAGTTCCT |
| Suggested primer 2: | GTGGTCATTGCCTCGGAGAA |