| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067728 |
| Chromosome: | chromosome 17 |
| Location: | 1644328 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707700 | NRM1,DMT1,NRAMP1 | (1 of 2) PTHR11706:SF33 - NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1; Manganese/metal transporter, NRAMP homolog | 3'UTR |
| Cre17.g707750 | (1 of 1) PTHR31040//PTHR31040:SF1 - FAMILY NOT NAMED // NURIM | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTACTGTTTTGAATGTAGTGAGCATGCATGCAATGTAAGCTTGTGCGA |
| Internal bar code: | GTTCGTGTGGTTCGTTTGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2108 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCCAGACCCAAGAGCTC |
| Suggested primer 2: | AGCCTGCTGCCATCAAATCT |