Insertion junction: CLIP2.067756_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.067756
Chromosome:chromosome 1
Location:3943742
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025900 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGATTCCGCCAGTTAAAGCGTTGCGGCCACGCGTTGCTGTGTGCATT
Internal bar code:GTGTCCAGAGGGCGCGAGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:464
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CATACTTCACCCCCACACCC
Suggested primer 2:AGGTGAATTGCGACGGACTT