| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067763 |
| Chromosome: | chromosome 16 |
| Location: | 2290143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658850 | (1 of 22) IPR018108//IPR023395 - Mitochondrial substrate/solute carrier // Mitochondrial carrier domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAGAGGCGCCAGCTCACCAGCTGGTGAATAGAAGTCGCGGATACAAT |
| Internal bar code: | TATTCCTTGACATGATGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4756 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGTGCAGTACAGCAGA |
| Suggested primer 2: | ATTGGGCCACAGAACGTCAT |