| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067770 |
| Chromosome: | chromosome 3 |
| Location: | 2101530 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156500 | (1 of 1) IPR000104//IPR016084 - Antifreeze protein, type I // Haem oxygenase-like, multi-helical | 3'UTR | |
| Cre03.g156550 | (1 of 1) PTHR22829//PTHR34826 - DEP DOMAIN PROTEIN // FAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCTTGACCCCGCTGCATGTCCTGCCATGTCTCCCTAGTCCTTGTGC |
| Internal bar code: | CCCGGGTGTAGCGTTAACGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2123 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGAGTTGGTTGCGGAGG |
| Suggested primer 2: | TTCCAGGATGCAGAGGAGGA |