| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067773 |
| Chromosome: | chromosome 4 |
| Location: | 4079708 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233302 | (1 of 2) PTHR23132//PTHR23132:SF9 - D-ALANINE--D-ALANINE LIGASE // SUBFAMILY NOT NAMED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAAATACCACGCACCTGAGGGGTGTGTGCGGGGTTCTGCACGAATTG |
| Internal bar code: | AATTAAGACAACTACTAGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4068 |
| LEAP-Seq percent confirming: | 89.5522 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTCAGGGCAAGTGTTG |
| Suggested primer 2: | TATCCAACCCGCCTAGACCA |