Insertion junction: CLIP2.067785_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.067785
Chromosome:chromosome 6
Location:3529552
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGTGAGATTTGAACCATTGCGGGTTCACCAAATTTGTTGATTTCCCG
Internal bar code:GGCGCGTCTGAGACGGATCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3620
LEAP-Seq percent confirming:86.8421
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGATTCACCCCCTGCATT
Suggested primer 2:AATGCGACCACCACTACCAG