| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067795 |
| Chromosome: | chromosome 2 |
| Location: | 4036933 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103000 | FKM3 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | outside_mRNA |
| Cre02.g103050 | (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGCGGCACCCGATGAGAAAACATACCTCTTTTCTCCTGCTGGCTA |
| Internal bar code: | ATAGATCATGGGTCCGCCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1171 |
| LEAP-Seq percent confirming: | 72.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCCGTCTAGCTTGACA |
| Suggested primer 2: | ACAGACGCCTATTTCGCACA |