Insertion junction: CLIP2.067805_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.067805
Chromosome:chromosome 9
Location:5580067
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g409700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACATGCACAATTGCGCCAGGTGGGGGAGGCATTCCGCTCTGGCA
Internal bar code:TCGTCCTCGTATGGTGCTGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:149
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTAACCCCTAACACACC
Suggested primer 2:GCGCCTGAGACTTATCCTCC