| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067812 |
| Chromosome: | chromosome 12 |
| Location: | 2763225 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504150 | NFU2,NIFU2 | Iron-sulfur cluster assembly protein; (1 of 1) PTHR11178//PTHR11178:SF1 - IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED // NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL | 3'UTR |
| Cre12.g504200 | RPS23 | (1 of 1) K02973 - small subunit ribosomal protein S23e (RP-S23e, RPS23); Cytosolic 80S ribosomal protein S23 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGAAAGGGCACGTAACCACCTCAACCGATCAGCCCATACGGCATAC |
| Internal bar code: | TAGTCTTCCGTATTCCCGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1939 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCTGCAAGCCGATTTGA |
| Suggested primer 2: | AGTGATTGGCGGGTGTTGAT |