| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067814 |
| Chromosome: | chromosome 9 |
| Location: | 579239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748347 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAATGATGCAAAACAACAGGATAGACGTAGCATGGGGCCATCAGGCCAT |
| Internal bar code: | CAGGAGTCACACGTGTGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGTTGCGGCTTGATTGG |
| Suggested primer 2: | TTACACAACGGCCGTGATGA |