| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067816 |
| Chromosome: | chromosome 9 |
| Location: | 5871175 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411800 | outside_mRNA | ||
| Cre09.g411850 | (1 of 2) PTHR23130:SF61 - DEFENSE PROTEIN L(2)34FC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCACCTGCACACGCTGCACAAAAGGGACGACCCCTTCCCGCCGCGGA |
| Internal bar code: | TGGATTTAGATCTACGCCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 5.55556 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCTGCAGAAGTACGGT |
| Suggested primer 2: | TCAGACAGCGAGAAACGTCC |