| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067823 |
| Chromosome: | chromosome 16 |
| Location: | 6368841 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675600 | (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCCGTTATCGGGAGGCGCGGCGCCAGGCCCAAGTGAGTCGCCCAGCC |
| Internal bar code: | AAGCAAAGGGATCCAGGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACACTTCCAGAGGTTG |
| Suggested primer 2: | ACGTGTGAGGTACAGCTGTG |