| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067829 |
| Chromosome: | chromosome 4 |
| Location: | 3511882 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228000 | (1 of 1) PF13890 - Rab3 GTPase-activating protein catalytic subunit (Rab3-GTPase_cat) | outside_mRNA | |
| Cre04.g228050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCAACGGTCATCATCGTTCACGACCGTTGGGGCGGTCAACCACTCTC |
| Internal bar code: | TGCGTATGACACCCCGCCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1350 |
| LEAP-Seq percent confirming: | 58.9744 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGGTGGTGATTGACAGA |
| Suggested primer 2: | ATGCTCTGGGTTGAGGATGC |