| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067831 |
| Chromosome: | chromosome 3 |
| Location: | 5194131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181450 | TCTN1,POB7 | (1 of 1) K19382 - tectonic-1/3 (TCTN1_3); Proteome of basal body 7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACCTGCATTGCCATACGAGGCTTGTGGTGACGCCTAACATTCACACG |
| Internal bar code: | TAAACACAAATGTTTAATAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2814 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGACTAGACCCGACACC |
| Suggested primer 2: | GCGTCATCATCGGCTTTGAC |