| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067833 |
| Chromosome: | chromosome 12 |
| Location: | 5867904 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533400 | (1 of 1) PTHR13527:SF0 - SAYSVFN DOMAIN-CONTAINING PROTEIN 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCACGCTCACTCACCACGCTGGCACACTAGCGGACCGCCATGCGATCC |
| Internal bar code: | CTGGTCAGTGTTTCGGCCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1734 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCACGTTCATGAGCAG |
| Suggested primer 2: | TCCATGCATCGTCCACAACA |