| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067841 |
| Chromosome: | chromosome 9 |
| Location: | 217163 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386746 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCCGAGTTTTGGCTCGAGAAAGTAGAGGTTCACAGATACAAGGATA |
| Internal bar code: | GCATTCGCGGAGGAGAACATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2430 |
| LEAP-Seq percent confirming: | 85.1562 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCAACCCTGTCATCGCTC |
| Suggested primer 2: | ATTTGCACACACGCTTCACC |