| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067844 |
| Chromosome: | chromosome 3 |
| Location: | 3824185 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169950 | (1 of 1) PF02373//PF02375 - JmjC domain, hydroxylase (JmjC) // jmjN domain (JmjN) | outside_mRNA | |
| Cre03.g170001 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCAAACTGGACCCCGTACTTCACACGTCTCACGAACAACCGTACAC |
| Internal bar code: | ACGGGGTTGTATGGAGCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2977 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGTTGTGTCAGCGTGT |
| Suggested primer 2: | GCCATGTGCATGATCACGAC |