| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.067849 |
| Chromosome: | chromosome 7 |
| Location: | 1046107 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319800 | (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATGCGAAGAAGTTTACGCTACTCATGACAGCTGCCCCACAACACTTC |
| Internal bar code: | CTCGTATAGGGCGCGGGCTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2388 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACAACTTCGACTTGCGTG |
| Suggested primer 2: | GGCTACGGCTCTAGCAGTTT |