Insertion junction: CLIP2.067851_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.067851
Chromosome:chromosome 14
Location:2436190
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g623650 ADH7 Alcohol dehydrogenase; (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTTCCCAACCTCCTCGAGGCAATGCTGCATGCTGCCCCGCACGCAACG
Internal bar code:CCACTATAGGGTGGGTGCCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGAACCCAGGCACGAGTTAA
Suggested primer 2:TCGGCTAACGCTTTCCCATT