| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.067854 |
| Chromosome: | chromosome 9 |
| Location: | 2657969 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801020 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCTCGCGTCAGCAGCATTCTCCAACTTTTTATCACGGCTAGGGCCAG |
| Internal bar code: | GTCGGGCTAGAGATTGGATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1400 |
| LEAP-Seq percent confirming: | 71.875 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGAAGCAGGCGTTTGTG |
| Suggested primer 2: | GGGTACTGCTAGTCTCACGC |