| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.067859 |
| Chromosome: | chromosome 7 |
| Location: | 1045409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319750 | HEL35 | (1 of 1) K14807 - ATP-dependent RNA helicase DDX51/DBP6 [EC:3.6.4.13] (DDX51, DBP6); DEAD box ATP-dependent RNA helicase | 3'UTR |
| Cre07.g319800 | (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAATCCGCACACATTGATTGAGTTCTGTACTTTGAGCGGCTTGCCG |
| Internal bar code: | TGATTGTGTTATTGTGTAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1194 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGAGACTCAACACCC |
| Suggested primer 2: | GGGTGATAGTTGGTTGGCGA |