Insertion junction: CLIP2.067869_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.067869
Chromosome:chromosome 9
Location:1391137
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGCCAGCCAGCCAGCCAGCCCGATATCCAGCCCACCAGTCCGATGTC
Internal bar code:CAGTACTACCGTAACTAATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:269
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTAGCGAGACTGCATCAT
Suggested primer 2:CTCGCAGTTTGATAACGCCG