| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067876 |
| Chromosome: | chromosome 11 |
| Location: | 2198260 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467726 | (1 of 1) K06316 - oligosaccharide translocation protein RFT1 (RFT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGATAATACCACCCGGCCAGACACCCGGCCACGCCGATGCGTCCTTCA |
| Internal bar code: | GTACAGGTGTGTTTGCTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3530 |
| LEAP-Seq percent confirming: | 96.8421 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 190 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATCAGCATAGGGGCCTG |
| Suggested primer 2: | CAATGAAGCCGCTCGTTAGC |