| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067886 |
| Chromosome: | chromosome 7 |
| Location: | 6103081 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355550 | (1 of 4) PTHR22911:SF6 - SOLUTE CARRIER FAMILY 35 MEMBER G1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCACCACATCCATACTAATTTGCCGCAAGCGACCGGAATCCTTACGG |
| Internal bar code: | AATAGTATGTAGGGATTTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1566 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCCGACAGTGAGAACA |
| Suggested primer 2: | TTGGTCAGGCAATACGGCAT |