| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067891 |
| Chromosome: | chromosome 10 |
| Location: | 2583264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436700 | (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | 3'UTR | |
| Cre10.g436800 | (1 of 2) PF01833//PF07691//PF10162 - IPT/TIG domain (TIG) // PA14 domain (PA14) // G8 domain (G8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAACGCTTGCCTAGCACTCGCCCCATTGCGCAGAGGCTTGGCTGGCA |
| Internal bar code: | AAGAACATAACTTACAACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTTGAAGCACGTACTGA |
| Suggested primer 2: | ATCTCCACTGGCAACGTCAG |