| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067894 |
| Chromosome: | chromosome 12 |
| Location: | 8799829 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGTCGGCGATGGCCGGATTCTGGCCGACCGACCGAATTTCGGCCACC |
| Internal bar code: | CCTTTTAAAGTCGATCTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2567 |
| LEAP-Seq percent confirming: | 93.4426 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCAACACAGTCGGCCC |
| Suggested primer 2: | ACAAGAGGACAACGCTCTGG |