| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067896 |
| Chromosome: | chromosome 2 |
| Location: | 7493592 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392616 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGAAACGCTGCAGTTTGTGCGGGGCACAACGCGTGCGAACCCGAAT |
| Internal bar code: | TCGATTACCTATTTACTACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1895 |
| LEAP-Seq percent confirming: | 60.7143 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCGTATAGTACTGCGT |
| Suggested primer 2: | CGCGGAGATGAGGGATAACC |