| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067901 |
| Chromosome: | chromosome 1 |
| Location: | 1737371 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800033 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGCGCCCTCACCTCGCCACTGCAGAACGCCACCAGTTCAGCGCGTT |
| Internal bar code: | ATTCTCTGGCGGAGAGGTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4571 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTCGTTGCTGCCGTCAA |
| Suggested primer 2: | GAAATGCTAGGATGCAGCGC |