| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.067904 |
| Chromosome: | chromosome 6 |
| Location: | 5027699 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281950 | (1 of 16) PF07707 - BTB And C-terminal Kelch (BACK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCAGTGGCCCGCAAGCGTGCCATTATGTGATCAAGTCATGCTTCAGC |
| Internal bar code: | CACGGTGCAACTAGTTGCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1783 |
| LEAP-Seq percent confirming: | 55.7692 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTGGTAGTTGTGGACG |
| Suggested primer 2: | AACTGGTACGGCATGGAAGG |