| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067906 |
| Chromosome: | chromosome 16 |
| Location: | 4790580 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687650 | ANK20 | Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGAATGCGGATGTTACAGAGGGGACAGTCCCAGCACCCGAAGACGCCG |
| Internal bar code: | CTCTGTTCAGGAAGGGCGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3943 |
| LEAP-Seq percent confirming: | 40.2174 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTTGGGACTGCACTGT |
| Suggested primer 2: | GTGAAAAGGCCCAAGCAAGG |