| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067909 |
| Chromosome: | chromosome 12 |
| Location: | 8446312 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCGTGTGAGGCACTACGGAGGCACGCCACACCTGGGCCATCCCGCG |
| Internal bar code: | TTGTCCATTAGTCATATCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1160 |
| LEAP-Seq percent confirming: | 21.0526 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACACGAAATTGCCACA |
| Suggested primer 2: | AACCCAAGCCCATGATGAGG |