| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067918 |
| Chromosome: | chromosome 13 |
| Location: | 1474523 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801520 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTTGGGGTATGAACTGGGGACACGGGCGGCCATACCAAACTACCAG |
| Internal bar code: | ATTTTATCCGTCGGGGCCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1437 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGACAGTTACGCGCCAGA |
| Suggested primer 2: | ACCACTGGATCAGGGCAATG |